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Analytical Chemistry (CHEM 333)
Analytical Chemistry (Chem 333, 3 credits) is typically taught in a fall semester and requires lab Chem 333L (1 credit) as a co-requisite. The value of analytical chemistry is in a wide range of applications. Various disciplines require information on the composition of the samples. This knowledge is for example needed in medical, environmental, forensic sciences as well as engineering fields. Even though, students who are taking this course will not perform the analysis in the future profession herself/himself. The understanding of the analytical methods is mandatory for the data interpretation, and the evaluation of the error of the method.
Who cares about data interpretation and errors? Imagine in medical field, the doctor will receive two lab results of 0.31 and 0.29, knowing that 0.30 is the threshold value. Thus, the doctor may conclude that patient with 0.31 has problem and needs a treatment while the second patient does not. However with analytical knowledge, the doctor also knows that error of the particular methods is 0.03. Therefore, he or she knows there is not really difference between results of those two patients and further follow-ups or tests are required.
In order to get strong understanding of analytical methods used, student will first review some sections of general chemistry such as stoichiometry, units of concentrations, titration, pH determination, equilibria. Students will also learn basics of statistical data evaluation, determination of error, and use of significant figures. This will be followed by specialized topics on electrochemistry, spectroscopy, chromatography and mass spectrometry, and also including sample preparation. It is important to understand that this class covers wide variety topics within one semester. To accomplish this course student need to take lab Chem 333L simultaneously. As this course is a junior (advanced) level, students need to expect that this implies also higher expectation in the laboratory. This may be seen in requirements on the accuracy and precision of the results as well as writing the reports.
Why accuracy? Consider the importance of accuracy on the following example: Analytical chemist would tell you that concentration of fluoride in your drinking water is 1 ppm (parts per million) which is currently level of fluoride recommended by EPA helping to prevent tooth decay. After few years you will find out there was an error in the accuracy and that actual concentration was 3 ppm (seemingly still very low), but causing particularly for children fluorosis – permanent alterations in teeth coloring.
- Analytical terminology - qualitative, quantitative, units
- Measurements tools & Statistics – accuracy, precision, error, deviation, significant digits, t-test, outliers, confidence int., P value
- Chemical equilibrium - review of general chemistry & constants K, Ksp, Ka, Kb, Kw, pH, solubility, neutralization, pKa, buffers
- Titration & Acid-base equilibrium, indicators
- Electrochemistry fundamentals – Oxidation/reduction, Nernst equation, potentials, pH electrode, electrodes, instrumental methods
- Spectrophotometry – molecular, absorption/emission AAS, AES, instruments
- Chromatography – separation, quantification, GC - injectors, columns, detectors, HPLC, IC
- Sample Preparation
- Mass Spectrometry – ionization, analyzer, instrumentation, interpretation
Quantitative Chemical Analysis textbook 8th edition By Daniel C. Harris
ISBN: 1-4292-1815-0, ISBN-13: 978-1-429-21815-3
- RF Turning Point Clicker (smartphone application available here)
- Calculator (capable of exponential function, non programmable) will be required for lecture, assignments and exams.
- Sapling homework website access
Materials for CHEM 333L:
- Safety goggles, paper towels, calculator, laboratory notebook (pages can be by hand)
- Laboratory Exercise Chemistry 333 will be provided